myc pvt1 copy number neutral cell lines  (ATCC)

Journal: bioRxiv

Article Title: Comparative analysis of wavelength-specific UV stress granule formation

doi: 10.64898/2026.03.15.711948

Figure Lengend Snippet: Representative images of SG formation by G3BP1 and TIA1 signals in wt U2OS cells treated as indicated. Cells were either untreated, treated with 250μM As III for 1h, or exposed to 15mJ/cm 2 of UV. UV samples were harvested and processed for imaging 4 hours after exposure. Yellow arrows indicate SGs. Scale bar = 20μm.

Article Snippet: Cultured cell lines U2OS (ATCC HTB-96), wild-type C57-Bl6 MEF (primary MEFs transformed by the 3T3 method, a gift of Joel D. Richter, UMass Chan Medical School) and HaCaT, were maintained at 37oC in a CO2 incubator (5% CO2) in DMEM (Gibco) supplemented with 10% FBS, 1% penicillin/streptomycin and 1% glutamine (0.2% glutamine for HaCaT cells).

Techniques: Imaging

Journal: bioRxiv

Article Title: Comparative analysis of wavelength-specific UV stress granule formation

doi: 10.64898/2026.03.15.711948

Figure Lengend Snippet: (A) Representative images of SG formation by G3BP1 and TIA1 signals in HaCaT cells treated as indicated. Cells were either untreated, treated with 250μM As III for 1h, or exposed to 15mJ/cm 2 of UV. UV samples were harvested and processed for imaging 4 hours after exposure. (B) Quantification of SG formation by G3BP1 and TIA1 signals in wt U2OS, HaCaT, and wt MEF cells treated as indicated. Cells were either untreated, treated with 250μM As III for 1h, or exposed to 15mJ/cm 2 of UV. UV samples were harvested and processed for imaging 4 hours after exposure. At least 250 cells and 3 fields of view were scored to calculate percent SG formation. n =3; error bars are ±SD; ns P > 0.05, ** P ≤ 0.01, **** P ≤ 0.0001 by one-way ANOVA and Dunnett’s post-hoc test. (C) Quantification of SG formation by G3BP1 and TIA1 signals in wt U2OS cells treated as indicated. Cells were either untreated, treated with 250μM As III for 1h, or exposed to 150mJ/cm 2 of UV. UV samples were harvested and processed for imaging 4 hours after exposure. At least 250 cells and 3 fields of view were scored to calculate percent SG formation. n =3; error bars are ±SD; ns P > 0.05, **** P ≤ 0.0001 by one-way ANOVA and Dunnett’s post-hoc test. Scale bar = 20μm.

Article Snippet: Cultured cell lines U2OS (ATCC HTB-96), wild-type C57-Bl6 MEF (primary MEFs transformed by the 3T3 method, a gift of Joel D. Richter, UMass Chan Medical School) and HaCaT, were maintained at 37oC in a CO2 incubator (5% CO2) in DMEM (Gibco) supplemented with 10% FBS, 1% penicillin/streptomycin and 1% glutamine (0.2% glutamine for HaCaT cells).

Techniques: Imaging

Journal: bioRxiv

Article Title: Comparative analysis of wavelength-specific UV stress granule formation

doi: 10.64898/2026.03.15.711948

Figure Lengend Snippet: (A-D) Representative images of SGs identified by G3BP1 co-stained with relevant SG markers (A) DHX9, (B) Rps6, (C) eIF3n, (D) Poly(A) fluorescence in situ hybridization (FISH). wt U2OS cells were either untreated, treated with 250μM As III for 1h, or exposed to 150mJ/cm 2 of UV. UV samples were harvested and processed for imaging 4 hours after exposure. Cells were stained with relevant SG marker antibodies and observed and imaged under 63X magnification. Scale bar = 20μm.

Article Snippet: Cultured cell lines U2OS (ATCC HTB-96), wild-type C57-Bl6 MEF (primary MEFs transformed by the 3T3 method, a gift of Joel D. Richter, UMass Chan Medical School) and HaCaT, were maintained at 37oC in a CO2 incubator (5% CO2) in DMEM (Gibco) supplemented with 10% FBS, 1% penicillin/streptomycin and 1% glutamine (0.2% glutamine for HaCaT cells).

Techniques: Staining, Fluorescence, In Situ Hybridization, Imaging, Marker

Journal: bioRxiv

Article Title: Comparative analysis of wavelength-specific UV stress granule formation

doi: 10.64898/2026.03.15.711948

Figure Lengend Snippet: Representative images of wt U2OS cells treated as indicated and stained for G3BP1 and Geminin. Cells were either untreated, treated with 250μM As III for 1h, or exposed to 150mJ/cm 2 of UV. UV samples were harvested and processed for imaging 4 hours after exposure. Yellow arrows indicate cells in S, G2, or M phase. Red arrows indicate cells in G1 phase. Scale bar = 20μm.

Article Snippet: Cultured cell lines U2OS (ATCC HTB-96), wild-type C57-Bl6 MEF (primary MEFs transformed by the 3T3 method, a gift of Joel D. Richter, UMass Chan Medical School) and HaCaT, were maintained at 37oC in a CO2 incubator (5% CO2) in DMEM (Gibco) supplemented with 10% FBS, 1% penicillin/streptomycin and 1% glutamine (0.2% glutamine for HaCaT cells).

Techniques: Staining, Imaging

Journal: bioRxiv

Article Title: Comparative analysis of wavelength-specific UV stress granule formation

doi: 10.64898/2026.03.15.711948

Figure Lengend Snippet: (A-B) Representative images (A) and quantification (B) of SG formation in wt U2OS cells after treatment as indicated. Cells were either untreated or pre-treated with 4sU for 1h. Following pretreatment cells were either untreated, treated with 250μM As III for 1h, or treated with UVA. UV samples were harvested and processed for imaging 8 hours after exposure. At least 250 cells and 3 fields of view were scored to calculate percent SG formation. n =4; error bars are ±SD; ns P > 0.05, * P≤0.05 by unpaired t test. Scale bar = 20μm.

Article Snippet: Cultured cell lines U2OS (ATCC HTB-96), wild-type C57-Bl6 MEF (primary MEFs transformed by the 3T3 method, a gift of Joel D. Richter, UMass Chan Medical School) and HaCaT, were maintained at 37oC in a CO2 incubator (5% CO2) in DMEM (Gibco) supplemented with 10% FBS, 1% penicillin/streptomycin and 1% glutamine (0.2% glutamine for HaCaT cells).

Techniques: Imaging

Journal: bioRxiv

Article Title: Comparative analysis of wavelength-specific UV stress granule formation

doi: 10.64898/2026.03.15.711948

Figure Lengend Snippet: (A) Representative images of wt U2OS cells stained for G3BP1 and keratin 8 treated as indicated. Cells were either untreated, treated with 250μM As III for 1h, or exposed to 150mJ/cm 2 of UV. UV samples were harvested and processed for imaging 4 hours after exposure. (B) Representative images of wt U2OS cells co-transfected with keratin 8 and keratin18_mCherry. Cells were either untreated, treated with 250μM As III for 1h, or exposed to 150mJ/cm 2 of UV. UV samples were harvested and processed for imaging 4 hours after exposure. (C) Quantification of SG formation in untransfected and co-transfected cells by G3BP1 staining. Cells were treated as indicated. At least 200 cells and 3 fields of view were scored to calculate percent SG formation. n =3; error bars are ±SD; ns P > 0.05, ** P ≤ 0.01, *** P ≤ 0.001 by unpaired t -test. Scale bar = 20μm.

Article Snippet: Cultured cell lines U2OS (ATCC HTB-96), wild-type C57-Bl6 MEF (primary MEFs transformed by the 3T3 method, a gift of Joel D. Richter, UMass Chan Medical School) and HaCaT, were maintained at 37oC in a CO2 incubator (5% CO2) in DMEM (Gibco) supplemented with 10% FBS, 1% penicillin/streptomycin and 1% glutamine (0.2% glutamine for HaCaT cells).

Techniques: Staining, Imaging, Transfection

Journal: bioRxiv

Article Title: Comparative analysis of wavelength-specific UV stress granule formation

doi: 10.64898/2026.03.15.711948

Figure Lengend Snippet: (A-B) Representative images (A) and quantification of SG formation in U2OS cells after treated as indicated. Cells were either untreated, treated with NAC (10mM) for 1h, treated with 250μM As III for 1h, co-treated with NAC and 250μM As III for 1h, treated with UV, or co-treated with UV and NAC. UV samples were harvested and processed for imaging 4 hours after exposure. At least 250 cells and 3 fields of view were scored to calculate percent SG formation. n =3; error bars are ±SD; ns P > 0.05, ** P ≤ 0.01, **** P ≤ 0.0001 by unpaired t -test.

Article Snippet: Cultured cell lines U2OS (ATCC HTB-96), wild-type C57-Bl6 MEF (primary MEFs transformed by the 3T3 method, a gift of Joel D. Richter, UMass Chan Medical School) and HaCaT, were maintained at 37oC in a CO2 incubator (5% CO2) in DMEM (Gibco) supplemented with 10% FBS, 1% penicillin/streptomycin and 1% glutamine (0.2% glutamine for HaCaT cells).

Techniques: Imaging